Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.135793 |
Chromosome: | chromosome 2 |
Location: | 191898 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTACACGATCAGTGCTGCGAGGAGTGAAT |
Internal bar code: | GTCGCGGTTTAGTCCCGCGTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 967 |
LEAP-Seq percent confirming: | 99.6545 |
LEAP-Seq n confirming: | 1442 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGTAGACAGGTGGTCGGGTG |
Suggested primer 2: | ACCGCCAACCATAGAAACAG |