| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135810 |
| Chromosome: | chromosome 16 |
| Location: | 4049556 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670050 | (1 of 1) PTHR10972//PTHR10972:SF69 - OXYSTEROL-BINDING PROTEIN-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAGCCCTCGTCCGCCGCATCCCGCCCAA |
| Internal bar code: | GTCCTGCCTTATGCGTGCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 843 |
| LEAP-Seq percent confirming: | 99.3939 |
| LEAP-Seq n confirming: | 328 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACTGCTTTGCTGGTGGCT |
| Suggested primer 2: | CTTCTTCTAGCAACCACGCC |