| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135824 |
| Chromosome: | chromosome 2 |
| Location: | 1698019 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g086000 | ARS8 | Arylsulfatase; (1 of 1) IPR002591//IPR003882//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Pistil-specific extensin-like protein // Alkaline-phosphatase-like, core domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATTACCTCCTGGGCAGCGCTGATGCCAA |
| Internal bar code: | AACATTAGTCTCCGATGTCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 99.7305 |
| LEAP-Seq n confirming: | 740 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGCTGGCAAACGTAAAT |
| Suggested primer 2: | CTATCGAAGGGAAACGGTCA |