| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.135844 |
| Chromosome: | chromosome 16 |
| Location: | 1373527 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTTCTCTCACTGGACTCCACAGACCGT |
| Internal bar code: | GGTCGCGTCACCTAGGCACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 99.5563 |
| LEAP-Seq n confirming: | 1122 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATTTGTGTGGGAAGAGA |
| Suggested primer 2: | AGCAATTACGGTATGGGCAG |