| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135858 |
| Chromosome: | chromosome 10 |
| Location: | 3804495 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446900 | (1 of 1) K12862 - pleiotropic regulator 1 (PLRG1, PRL1, PRP46) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGGTGGTGACCCTGGGTGGTGACGGGC |
| Internal bar code: | GTAGGGATTCTTTCTTTCTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 604 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGGGGCTCTGTATGCCAC |
| Suggested primer 2: | GATTTGGACTTCACGTCGGT |