| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135880 |
| Chromosome: | chromosome 6 |
| Location: | 5923902 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGATCTCTCGTTCTGTGGCTGGCGTGG |
| Internal bar code: | TAATGTTTTTTCGTTTTAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 99.0625 |
| LEAP-Seq n confirming: | 317 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAGACATAGCAGGTTGCG |
| Suggested primer 2: | ACCGTATGCCGTCAAAACTC |