| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135891 |
| Chromosome: | chromosome 10 |
| Location: | 475821 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421000 | (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTATCGCTGTGTCTCTGTGTGGCTGTGTA |
| Internal bar code: | ACCCTCGGAGTGGTCAGCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 98.6618 |
| LEAP-Seq n confirming: | 1622 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGTGTGTGTATTTCCGT |
| Suggested primer 2: | GATATCCTCACCCAGCCTGA |