Insertion junction: LMJ.RY0402.135937_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.135937
Chromosome:chromosome 12
Location:725060
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g495100 PSR1 (1 of 1) PF00249//PF14379 - Myb-like DNA-binding domain (Myb_DNA-binding) // MYB-CC type transfactor, LHEQLE motif (Myb_CC_LHEQLE); Phosphorus starvation response 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTCAAAACTTGCTCGCGCTCCCTTGGCG
Internal bar code:GGAGTTGAAGACACTAGTACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:197
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGTCCCATCACACACTTG
Suggested primer 2:ATGCCCGTTTGCATAAAGTC