| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135954 |
| Chromosome: | chromosome 12 |
| Location: | 3110620 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACATCCACATCTTCACCGCTCTCGACT |
| Internal bar code: | ATGTTATCCCACTCTTGTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 338 |
| LEAP-Seq percent confirming: | 35.2941 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACAGAATGCTGGACTCGG |
| Suggested primer 2: | CTCGTGGGACAATGTGTTTG |