| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135955 |
| Chromosome: | chromosome 1 |
| Location: | 602565 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003487 | (1 of 1) K13106 - pre-mRNA-splicing factor CWC26 (BUD13, CWC26) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACAGTCGGTCCAAATCGGTTCGGAAGGC |
| Internal bar code: | AGGTCTACATATCGATTCGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 246 |
| LEAP-Seq percent confirming: | 99.5912 |
| LEAP-Seq n confirming: | 3654 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTCCTGTGGTGTCGGACT |
| Suggested primer 2: | GCTCCTTGCAAACACAGTGA |