| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.135970 |
| Chromosome: | chromosome 15 |
| Location: | 881473 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g637850 | OPR68 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTCAACACTCCTGGCGCGACAACGCCT |
| Internal bar code: | GGTTGACCGTGCAGTCAAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 98.7903 |
| LEAP-Seq n confirming: | 735 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTACTAAGCACACGCCTC |
| Suggested primer 2: | ACAAGACTGTGCAAACACGC |