| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135974 |
| Chromosome: | chromosome 6 |
| Location: | 2898878 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272300 | CPLD57 | (1 of 1) PTHR23029:SF45 - PHOSPHOGLYCERATE MUTASE FAMILY PROTEIN; Phosphoglycerate mutase family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCATGACAGGCCTGTCGGTTCGCAGTGG |
| Internal bar code: | GAACCCGACACCGGACCGACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 822 |
| LEAP-Seq percent confirming: | 94.5985 |
| LEAP-Seq n confirming: | 3240 |
| LEAP-Seq n nonconfirming: | 185 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGGGCATTTTTGCGACAC |
| Suggested primer 2: | ATCAAAAATACGCCTGGTGC |