| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135977 |
| Chromosome: | chromosome 12 |
| Location: | 299894 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485600 | SNRK2M | (1 of 4) PTHR24343//PTHR24343:SF167 - SERINE/THREONINE KINASE // SUBFAMILY NOT NAMED; snRK1 family in Chlamydomonas, subgroup 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGGGCACAGAGCCACGCCTGGCCCACG |
| Internal bar code: | CAGCCTCAATGGCGGCATTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 99.8348 |
| LEAP-Seq n confirming: | 6043 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTTGCGTGGTCAGTACA |
| Suggested primer 2: | GGTCAGGGTCATCTACGCAT |