| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135986 |
| Chromosome: | chromosome 10 |
| Location: | 4345816 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTTGAGACAAACGCCTGCGGCCTACCA |
| Internal bar code: | ATGATAACGCAAACGCCCGGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 903 |
| LEAP-Seq percent confirming: | 99.1047 |
| LEAP-Seq n confirming: | 2214 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGGTATTGTGTGGTGCG |
| Suggested primer 2: | AACTACCGTGTTACGCCCTG |