| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135989 |
| Chromosome: | chromosome 13 |
| Location: | 1218529 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570250 | CKIN1L,SNRK1B,CKIN2 | (1 of 1) PF07714//PF14531 - Protein tyrosine kinase (Pkinase_Tyr) // Kinase-like (Kinase-like); snRK1 family in Chlamydomonas, subgroup 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGAAGTGGGGAAGAGAGAGGGCAGGAA |
| Internal bar code: | TGATCATCGTACGGTGCATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 819 |
| LEAP-Seq percent confirming: | 99.797 |
| LEAP-Seq n confirming: | 2458 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGGTCCCAATAGGGGTGG |
| Suggested primer 2: | TGCCGATGAAATCTTAAGGG |