Insertion junction: LMJ.RY0402.136003_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.136003
Chromosome:chromosome 5
Location:2943373
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g238260 (1 of 1) PF13371//PF13414//PF13432 - Tetratricopeptide repeat (TPR_9) // TPR repeat (TPR_11) // Tetratricopeptide repeat (TPR_16) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAGTTTGAAGCGGGGAGGGAGGTTAGAC
Internal bar code:ATGTACGTTATCGGCGGTTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:302
LEAP-Seq percent confirming:99.9439
LEAP-Seq n confirming:8906
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCCTGTCCCTGTCCCT
Suggested primer 2:AATCAGAGGGCACTCGCTAA