| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.136004 |
| Chromosome: | chromosome 7 |
| Location: | 5020881 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347100 | (1 of 1) PTHR11122:SF11 - ALDOSE 1-EPIMERASE FAMILY PROTEIN-RELATED; Putative sugar epimerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCAAGGCGTCCTCCCTCCACGGCCGCGT |
| Internal bar code: | TTGCTTACCCTCTTCTTGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 463 |
| LEAP-Seq percent confirming: | 98.8372 |
| LEAP-Seq n confirming: | 1445 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | AGTCGGAAGACCAGCTTTCA |