| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.136034 |
| Chromosome: | chromosome 2 |
| Location: | 6319954 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g114900 | ANK23 | Predicted protein with ankyrin repeats; (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCTCGGCGCGCTTGCTGGCTGAACTTGT |
| Internal bar code: | CTGGGTCAGAAAACGTTTCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 338 |
| LEAP-Seq percent confirming: | 99.1485 |
| LEAP-Seq n confirming: | 1048 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTGCAGGGTCTGACTTG |
| Suggested primer 2: | CGGGTTTGTGATTGTGTCAG |