Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.136090 |
Chromosome: | scaffold 40 |
Location: | 9507 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre40.g760197 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCCGCTGTGGGGCAAGGTGGCCGATGC |
Internal bar code: | TTCGCACCCGTCGGAGTACCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 300 |
LEAP-Seq percent confirming: | 99.5486 |
LEAP-Seq n confirming: | 1985 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATGATCCTACATGGACCCC |
Suggested primer 2: | GCACTTTTCCTTTGTCCAGC |