| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.136100 |
| Chromosome: | chromosome 7 |
| Location: | 1139988 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g320500 | OPR29 | OctotricoPeptide Repeat protein 29 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCAGGTAACGACGGGATAACTCAGGTGC |
| Internal bar code: | AGGTGGTGCTATAGCTCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 99.9274 |
| LEAP-Seq n confirming: | 1377 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTTTATTCACCGTGGCT |
| Suggested primer 2: | CGTAGAACTCCGACAGCCAT |