Insertion junction: LMJ.RY0402.136101_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.136101
Chromosome:chromosome 13
Location:2536369
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g580600 ATT1,ATE1 Arginyl-tRNA protein transferase; (1 of 1) 2.3.2.8 - Arginyltransferase / Arginyl-tRNA--protein transferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGGCGTGTGTCACATGTGTGTTGGACAA
Internal bar code:GAACGGGACGGCGTGGTGCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:492
LEAP-Seq percent confirming:79.5033
LEAP-Seq n confirming:11365
LEAP-Seq n nonconfirming:2930
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTACGTCAGGTGGGTGTG
Suggested primer 2:CCCACACTACACACACTCCG