Insertion junction: LMJ.RY0402.136105_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.136105
Chromosome:chromosome 12
Location:7017287
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g559750 PRL2 (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED; Predicted extracellular protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCGGTGACACCGAGGCGGCTGCCGGTCC
Internal bar code:AGCTGCGGTCCCGCCTTGTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:281
LEAP-Seq percent confirming:94.8787
LEAP-Seq n confirming:2112
LEAP-Seq n nonconfirming:114
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGACGTCATTCAGCTTCA
Suggested primer 2:GGCTGGATCCGTGTTACAGT