Insertion junction: LMJ.RY0402.136120_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.136120
Chromosome:chromosome 17
Location:7018475
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g746597 (1 of 2) K16296 - serine carboxypeptidase-like clade I [EC:3.4.16.-] (SCPL-I) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGGAGGGGGAGGGGTCACCGCTCCCGTC
Internal bar code:TGTATCTTGGAGGACGCCCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:194
LEAP-Seq percent confirming:92.4264
LEAP-Seq n confirming:1318
LEAP-Seq n nonconfirming:108
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACAACAAACCTCACCATCG
Suggested primer 2:GTCGTCCATATGTGCAGTGG