| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136176 |
| Chromosome: | chromosome 10 |
| Location: | 281567 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419800 | PIS1 | (1 of 1) K00999 - CDP-diacylglycerol--inositol 3-phosphatidyltransferase (CDIPT); Phosphatidylinositol synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCTCCAGGAGATTCACAGGCGCAGGCAC |
| Internal bar code: | CCGCTCCTGCACGCCACGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 838 |
| LEAP-Seq percent confirming: | 99.8653 |
| LEAP-Seq n confirming: | 2225 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGACTGATGGCTTTGCGTA |
| Suggested primer 2: | TGGGAACTTCGGTAAAGTGG |