| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136176 |
| Chromosome: | chromosome 10 |
| Location: | 281591 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419800 | PIS1 | (1 of 1) K00999 - CDP-diacylglycerol--inositol 3-phosphatidyltransferase (CDIPT); Phosphatidylinositol synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATACACCATGCACCGCCCCATACCCGCT |
| Internal bar code: | GATGGCGCAAGGGACGGAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 856 |
| LEAP-Seq percent confirming: | 63.4058 |
| LEAP-Seq n confirming: | 175 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAACTTCGGTAAAGTGG |
| Suggested primer 2: | CAGACTGATGGCTTTGCGTA |