Insertion junction: LMJ.RY0402.136176_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.136176
Chromosome:chromosome 12
Location:725055
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g495100 PSR1 (1 of 1) PF00249//PF14379 - Myb-like DNA-binding domain (Myb_DNA-binding) // MYB-CC type transfactor, LHEQLE motif (Myb_CC_LHEQLE); Phosphorus starvation response 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAACTTGCTCGCGCTCCCTTGGCGGAGCT
Internal bar code:CTGACGAGGGACCCCGGTGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:975
LEAP-Seq percent confirming:99.8287
LEAP-Seq n confirming:2914
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCCCGTTTGCATAAAGTC
Suggested primer 2:CCTGTCCCATCACACACTTG