| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136226 |
| Chromosome: | chromosome 10 |
| Location: | 3959711 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448550 | (1 of 3) PF14240 - YHYH protein (YHYH) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCACTAAGGTGTCCAATGCGGCCTGCAC |
| Internal bar code: | CGGCTTGGATCAGACCTAACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 922 |
| LEAP-Seq percent confirming: | 99.8929 |
| LEAP-Seq n confirming: | 1865 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTAGTAGCGGCAGCCAT |
| Suggested primer 2: | GCATGAGTGCGTAGGACAGA |