| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136299 |
| Chromosome: | chromosome 3 |
| Location: | 7270825 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207351 | (1 of 2) PF10998 - Protein of unknown function (DUF2838) (DUF2838) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGCCCGAACTCATCAACACAGCCTCTAA |
| Internal bar code: | AAGCAGGGGCGGAGTACTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 99.3789 |
| LEAP-Seq n confirming: | 480 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTACGGGTGACCATTTCCA |
| Suggested primer 2: | CGCAAGTAAGACAGGAAGGC |