Insertion junction: LMJ.RY0402.136303_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.136303
Chromosome:chromosome 4
Location:2740460
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g223550 (1 of 26) IPR011990 - Tetratricopeptide-like helical domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAACTCCCACCCTTCCCTCGCTCACCCG
Internal bar code:CAACGGAGGATTAAAGGTATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:670
LEAP-Seq percent confirming:93.9394
LEAP-Seq n confirming:2015
LEAP-Seq n nonconfirming:130
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CGATTCCTTGATTCTTGGGA
Suggested primer 2:CCTCACCAACAAGTGGGTCT