| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136305 |
| Chromosome: | chromosome 9 |
| Location: | 2767977 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389100 | GOT1 | (1 of 3) PF04178 - Got1/Sft2-like family (Got1); Got1, COPII vesicle component | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGGCGCATACATGGGGACCAGGAGGTT |
| Internal bar code: | CCGGCCACAGTCCCAGGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 441 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 271 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAAAGGAGCGGAGCACAG |
| Suggested primer 2: | GCCTGGTGGTTTTATGCACT |