Insertion junction: LMJ.RY0402.136346_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.136346
Chromosome:chromosome 2
Location:1598998
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g085050 COP1,PCOP1 Plant-type constitutive photomorphogenesis protein 1; (1 of 2) K10143 - E3 ubiquitin-protein ligase RFWD2 (RFWD2, COP1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTGGTCGGCTACAGGACTCATCAGCGCC
Internal bar code:CATGTTTGAGTGTCGAGGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:704
LEAP-Seq percent confirming:75.9602
LEAP-Seq n confirming:1068
LEAP-Seq n nonconfirming:338
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTGTCTTTGGTGGTCTTT
Suggested primer 2:CCCCAGTTGTCACCTTGAGT