Insertion junction: LMJ.RY0402.136361_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.136361
Chromosome:chromosome 12
Location:7800996
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g553500 ANK15 Predicted protein with ankyrin repeats; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCGGGTTTTGAACAGTCGGCACGAAGG
Internal bar code:GGGGGGGGCTCTGATCTTAGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:600
LEAP-Seq percent confirming:97.9592
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCACACGGCATTCTACTC
Suggested primer 2:AAAGCATGGATAAGCGTTCG