| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.136368 |
| Chromosome: | chromosome 16 |
| Location: | 955399 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648700 | (1 of 4) 3.6.3.29 - Molybdate-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAGGTGGACCAGCTCCTGGACGCCCTCG |
| Internal bar code: | TGATCCGTTTGGAATATTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 96.2543 |
| LEAP-Seq n confirming: | 848 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACGACATGAAGTTGGGG |
| Suggested primer 2: | AGTCCCGTGCACATACACAA |