| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136370 |
| Chromosome: | chromosome 17 |
| Location: | 2944362 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719900 | PWD1 | Phosphoglucan water dikinase 1; (1 of 1) K15535 - phosphoglucan, water dikinase (PWD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGGAGGAGGGGATTCTCGTGCCGCGTG |
| Internal bar code: | TCGAAGAGGCCCCAATGTCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 881 |
| LEAP-Seq percent confirming: | 98.8739 |
| LEAP-Seq n confirming: | 1317 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCCTGACCCTCTTTACA |
| Suggested primer 2: | ATGACAATTTCCGAGGTTCG |