| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136392 |
| Chromosome: | chromosome 9 |
| Location: | 3185867 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387282 | (1 of 1) PTHR13600//PTHR13600:SF22 - LEUCINE CARBOXYL METHYLTRANSFERASE // S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCAGGGGTTAGAACAAGGAGCTTGCTA |
| Internal bar code: | AGGAAGAACGGGGCGACTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 55 |
| LEAP-Seq percent confirming: | 13.3333 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTCAGGCGTCTTTGAGT |
| Suggested primer 2: | GCACACACCATCAACACACA |