| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136398 |
| Chromosome: | chromosome 6 |
| Location: | 5081904 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280600 | GEX1 | Gamete-expressed protein 1; (1 of 1) PTHR33538:SF2 - PROTEIN GAMETE EXPRESSED 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGAGCTTTCTGCAAGCATTGTTCGGCA |
| Internal bar code: | AAACTGGTGTAGACTGTTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 801 |
| LEAP-Seq percent confirming: | 99.2683 |
| LEAP-Seq n confirming: | 3663 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTGAAATCCTCGCACAG |
| Suggested primer 2: | CAAGGAAGGGAAGATGTGGA |