| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136417 |
| Chromosome: | chromosome_9 |
| Location: | 3271018 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g387986 | antisense | 3'UTR|outside_mRNA | ||
| Cre09.g388023 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGACACCTACACCGCGACTACACAAGCACG |
| Internal bar code: | TCGCGTCACAGACTGACAGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 94.3284 |
| LEAP-Seq n confirming: | 316 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACACTGGCAGAACTGGA |
| Suggested primer 2: | CATATGCGTCACAACTGCCT |