Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.136428 |
Chromosome: | chromosome_2 |
Location: | 3761705 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g095300 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTATCCGTGTGCGTGCGACTCCAGGGCACC |
Internal bar code: | ACGTATTGACAGCCGGCAAATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 357 |
LEAP-Seq percent confirming: | 99.7225 |
LEAP-Seq n confirming: | 2156 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGTTGGCAAGGGTTTATTT |
Suggested primer 2: | TGACGCTATTTTGTTGCTGC |