Insertion junction: LMJ.RY0402.136434_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.136434
Chromosome:chromosome 17
Location:6811035
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g744997 PNO4 FAD dependent oxidoreductase; (1 of 1) PTHR10617//PTHR10617:SF52 - ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE // OXIDOREDUCTASE/ELECTRON CARRIER-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGATGTGCGCATTGCCAGCTTAGGCAATC
Internal bar code:CTACTATTGAGGCCCCTCGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:106
LEAP-Seq percent confirming:99.3976
LEAP-Seq n confirming:165
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAAGCTTATCCAAACCAA
Suggested primer 2:GTGTGCGCGTGTTGTGTATT