| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136439 |
| Chromosome: | chromosome 5 |
| Location: | 769108 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248450 | CAH5 | Mitochondrial carbonic anhydrase; (1 of 3) PTHR11002//PTHR11002:SF17 - CARBONIC ANHYDRASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTGTGCTAAGGGGCGGGGGGCGCTGCT |
| Internal bar code: | CATAACGGATTTCGTACCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 96.1589 |
| LEAP-Seq n confirming: | 2954 |
| LEAP-Seq n nonconfirming: | 118 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGCAAGCGTTTTTGATT |
| Suggested primer 2: | TGTACCAAGCCAAACATCCA |