| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136444 |
| Chromosome: | chromosome 12 |
| Location: | 3425714 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCCTGATGTCTCACCTGCAAGCAAGGC |
| Internal bar code: | CCGATGCGATTTCGCATAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 96.363 |
| LEAP-Seq n confirming: | 26760 |
| LEAP-Seq n nonconfirming: | 1010 |
| LEAP-Seq n unique pos: | 101 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAAAGGTGCTCTTCGGTG |
| Suggested primer 2: | GTCCTCCCTGTAACGCTCTG |