| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136446 |
| Chromosome: | chromosome_7 |
| Location: | 1956838 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g325751 | FAP258 | Similar to Flagellar Associated Protein FAP258 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGCCCCCCACGCTCAGCCCAGCCAGCCGC |
| Internal bar code: | AACGCCCTCAGGAGTACCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 241 |
| LEAP-Seq percent confirming: | 99.5204 |
| LEAP-Seq n confirming: | 415 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTTTGGCTCATTGCATA |
| Suggested primer 2: | ACAGGGACAGGGACACAAAG |