| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.136449 |
| Chromosome: | chromosome 5 |
| Location: | 1198075 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245451 | RBL10 | Rhomboid-like protease; (1 of 11) 3.4.21.105 - Rhomboid protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGCCCCTACCACGCCCGTCTACTTCCA |
| Internal bar code: | TAAGTTTGGCTGGGTTACTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1031 |
| LEAP-Seq percent confirming: | 99.8701 |
| LEAP-Seq n confirming: | 1538 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGAATCCCTCCGTTCTCC |
| Suggested primer 2: | ACGGGTACTTCCTCGTGATG |