| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136455 |
| Chromosome: | chromosome 1 |
| Location: | 4533143 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030900 | (1 of 1) 6.2.1.26 - o-succinylbenzoate--CoA ligase / OSB-CoA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATACCGGTGTTGGGCGGCTTGTATCCCAG |
| Internal bar code: | CGGATCCCGGGACGAATATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 661 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGGGGAGGACATCAGAG |
| Suggested primer 2: | GTCAGCCTTCACGTGCTGTA |