| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136468 |
| Chromosome: | chromosome 13 |
| Location: | 553371 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565301 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCCATGTGCATACCTGGTGGTTTAGAA |
| Internal bar code: | TCGATCGCGCCTAGGCCCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 745 |
| LEAP-Seq percent confirming: | 93.7065 |
| LEAP-Seq n confirming: | 2695 |
| LEAP-Seq n nonconfirming: | 181 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAGGGAGGAGGTCATGT |
| Suggested primer 2: | GTGTATTGGAGCTCGGGTGT |