| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136509 |
| Chromosome: | chromosome 4 |
| Location: | 3949141 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g231222 | CPN60A,CPN60 alpha | Chaperonin 60A; (1 of 1) PTHR11353:SF11 - CHAPERONIN 60 SUBUNIT ALPHA 1, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTGTGTGGGCACCTGCCGGAGCACGCC |
| Internal bar code: | ATGAGATACGCGTCGAGCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 357 |
| LEAP-Seq percent confirming: | 97.3008 |
| LEAP-Seq n confirming: | 757 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCAGGAATGACCAGAGAC |
| Suggested primer 2: | GAACCCCACTGCAACAGAGT |