| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136513 |
| Chromosome: | chromosome_11 |
| Location: | 877482 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g467653 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTGCGGGACGAGCAGCAGCGGTGCTGCCC |
| Internal bar code: | CGGTCGGAAGGGGTTCATGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 621 |
| LEAP-Seq percent confirming: | 98.9324 |
| LEAP-Seq n confirming: | 834 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGACCAACGTCTTCACC |
| Suggested primer 2: | CGAAACGAACAGATGAAGCA |