| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.136531 |
| Chromosome: | chromosome 15 |
| Location: | 781499 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640101 | (1 of 3) IPR000104//IPR006553 - Antifreeze protein, type I // Leucine-rich repeat, cysteine-containing subtype | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAACGGCTTAACCGCCACACCTGCGCCG |
| Internal bar code: | CCTTAAACGTCCTTCCTCCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 247 |
| LEAP-Seq percent confirming: | 97.7974 |
| LEAP-Seq n confirming: | 1332 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACAAAGCGTTCGGTAAC |
| Suggested primer 2: | GAAGCGATATGGGAGGTGAG |