| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136535 |
| Chromosome: | chromosome 17 |
| Location: | 6239642 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g742250 | NCG9,CDKG2 | Cyclin dependent kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGTGTTTTGCACGGCATGCCATGCGAC |
| Internal bar code: | GTCGTAGAGTGTGCAGGGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 716 |
| LEAP-Seq percent confirming: | 99.7724 |
| LEAP-Seq n confirming: | 2192 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGTGGCCTGTCTTACCC |
| Suggested primer 2: | AGGGCGTACGTGATTTGTTC |