| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136537 |
| Chromosome: | chromosome 1 |
| Location: | 5635357 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040100 | (1 of 1) PF13902 - R3H-associated N-terminal domain (R3H-assoc) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGGGAGATGGGCCGGGCGCTGCTCTGG |
| Internal bar code: | AGGTCTATTCGGAAGGACAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 94.0299 |
| LEAP-Seq n confirming: | 2646 |
| LEAP-Seq n nonconfirming: | 168 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGCAACTAGGAGTTTGG |
| Suggested primer 2: | AATGCAGTGCAAGACGAGTG |